This volume describes numerous applications of sponge-matrix hisulture to study cancer biology and the treatment of cancer, stem cells, organoids, growth and repair of nerves, lymphoid tissues that produce antibodies, and HIV infection. The chapters in this book cover topics including, in vivo-like growth patterns of multiple types of tumors in Gelfoam® hisulture; development of the hisulture drug response assay (HDRA) for cancer patients; hair-shaft growth in Gelfoam® hisulture of skin as well as isolated hair follicles in Gelfoam® hisulture; the use of Gelfoam® hisulture to discover novel treatment of HIV infection; and imaging DNA repair after UV irradiation damage of cancer cells in Gelfoam® hisulture. The volume explains what is true 3D cell and tissue culture and corrects widespread misconceptions of 3D culture in the literature. Written in the highly successful
Methods in Molecular Biology
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.
Cutting–edge and authoritative,
3D Sponge-Matrix Hisulture: Methods and Prools
is a valuable resource that contributes to new cell and tissue culture research, and other diseases based on the true 3D culture.