In contemporary research, zebrafish (Danio rerio) have emerged as a pivotal model organism due to their genetic similarities with humans, ease of genetic manipulation, and high reproductive rate. These attributes make them ideal for studies in toxicology and gerontology. This guide aims to provide comprehensive insights into the handling, maintenance, and experimental procedures involving zebrafish, ensuring accurate and reproducible results in scientific investigations. By adhering to these guidelines, researchers can effectively utilize zebrafish in understanding the impacts of various substances on health and aging, contributing to advancements in biomedical research.