Presenting the latest developments in the field for more than four decades, the Advances in Chromatography series is relied on by scientists and researchers for the most up-to-date information on a wide range of chromatographic methods and applications. Volume 52 continues this tradition with contributions by established, well-known chemists, offering cutting-edge reviews of chromatographic methods with applications in the life sciences.Featured topics include The history, development, and theory behind aerosol-based detectors Protein and peptide biomarkers that are chromatographic endpoints measured in biological fluids and tissues, such as serum, urine, and tissue biopsies Multisegment linear gradient optimization strategy in reversed-phase and hydrophilic interaction chromatographic systems Enantioselective gas chromatography performed using three distinct chiral stationary phases based on hydrogen bonding, metal ion complexation, and inclusion Analysis of the dynamic phenomena of liquid chromatographic systems with six typical reactions in the mobile phase Providing a clear presentation of topics and vivid illustrations for which this series has become known, the latest volume makes the material accessible and engaging to analytical, biochemical, organic, polymer, and pharmaceutical chemists at all levels of technical skill.