A fuel that is produced from biomass is referred to as biofuel. It primarily includes the liquid or gaseous fuels that are used for transportation. It is considered as a form of renewable or green energy if the biomass used in the production of biofuel regrows quickly. Biodiesel and bioethanol are the most common types of biofuels. Biodiesel is manufactured from oils or fats through transesterification. It is often used in vehicles as a fuel as well as a diesel additive for the reduction of carbon-monoxide and hydrocarbons from diesel-powered vehicles. Bioethanol is produced by fermentation. It is made up of carbohydrates that are produced in sugar or starch crops like sugarcane and corn. This book unfolds the innovative aspects of biofuels which will be crucial for the holistic understanding of the subject matter. It elucidates new techniques and their applications in a multidisciplinary approach. This book will serve as a valuable source of reference for those interested in this field.