This second edition provides new and updated tools for studying protein—carbohydrate interactions ranging from traditional biochemical methods to state—of—the—art techniques. This book focuses on four different research themes detailing methods for screening and quantifying CAZyme activity, investigating the interactions between proteins, carbohydrate ligands, methods for the visualization of carbohydrates, protein—carbohydrate complexes, structural and “omic” approaches for studying systems of CAZymes. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step—by—step, readily reproducible prools. Authoritative and cutting—edge, Carbohydrate— Protein Interactions: Methods and Prools, Second Edition aims to be comprehensive guide for researchers inthe field.