Growing cells in 2D under static conditions has long been the gold standard of cell culture, despite this method not being representative of the complex
in vivo
environment. Focusing on new 3D
in vitro
methods now available to researchers, this book brings together examples of leading-edge work being conducted internationally for improving
cell culture methods, in particular the use of systems for enabling cell culture under laminar flow and the use of 3D scaffolds for providing cells with a structure which replicates the function of the extracellular matrix and encouraging interactions more akin to an
environment.