Many advances in modern neuroscience are enabled by the availability of chemical tools that allow sensitive, precise, and quantitative measurements of, and control over, biological processes. These powerful reagents are widely used for investigating the nervous system at levels of detail ranging from ion channel structure to neural network dynamics. Recent advances in phohemistry, microscopy, and protein engineering have triggered a surge in the development and application of these interdisciplinary techniques.
Chemical Neurobiology: Methods and Prools
assists with the design, characterization and validation of new chemical tools for neurobiology by providing detailed prools of procedures and assays deemed essential for the successful development and implementation of such tools. Divided into three sections, topics cover chemical probes of membrane protein structure and function, phohemical control of protein and cellular function, and chemical probes for imaging in the nervous system. Written in the successful
Methods in Molecular Biology™
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible prools, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible,
serves scientists at many levels, including students aiming to expand their perspective, laboratory researchers seeking technical guidance, and established investigators looking for creative solutions to their research problems in molecular, cellular and systems neuroscience.