“Next generation” sequencing techniques allow for more detailed analysis of exons and introns in multiple genes at the same time. This will reveal many mutations that potentially lead to exon skipping. To functionally test these a lot can be achieved with a limited set of prools, while for the intentional induction of exon skipping different tools and target genes are involved and the translational path from
in vitro
splicing to
in vivo
tests in animal models requiring a more extensive set of prools.
Exon Skipping: Methods and Prools
provides scientist with a comprehensive guide to many of the methods and techniques used for exon skipping, such as methods on how to discriminate “real polymorphisms” from mutations that affect splicing. Written in the highly successful
Methods in Molecular Biology™
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical
seeks to aid scientists in the continuing study of exon skipping.