This book focuses on the applications of extracellular vesicles (EVs) for regenerative medicine. EVs are small, lipid-bound vesicles carrying cargo that can be released by virtually all cell types. Over the past decades, the field of EV research has experienced exponential growth. Once dismissed as mere cellular debris, EVs are now recognized as sophisticated messengers, facilitating the transmission of crucial information between cells and orchestrating a myriad of biological processes. Notably, stem cell-derived EVs hold particular promise in regenerative therapeutic applications. Rich in the functional components of stem cells, these EVs offer an alternative approach to cell therapy for tissue repair and regeneration. The purpose of this book is to provide an overview of EVs on their biology, function, and potential applications. The book starts with exploring the intricate biology of EVs, their cargo, and their unique ability to modulate cellular processes. It discusses the therapeutic potential of EVs in various diseases, as well as the approaches for the bionanotechnological development of artificial EVs for theranostics. Large-scale EV isolation methods and their preclinical applications are also addressed. It will be useful for undergraduate and graduate students in medicine and cell biology, biologist, and others who are interested in such topic.