Carotenoids are a family of yellow to orange-red terpenoid pigments synthesized by photosynthetic organisms and many bacteria and fungi. They have beneficial health effects protecting against oxidative damage, and may be responsible for the colours associated with plants and animals. In
Microbial Carotenoids From Fungi: Methods and Prools,
expert researchers in the field detail many of the most up-to-date methods which are now commonly used to study carotenoids. These include methods for the manipulation and metabolic engineering of the Beta-carotene producing fungi
Blakeslea trispora
and
Mucor circinelloides
, lycopene production with the yeast
Yarrowia lipolytica
, peroxisome targeting of lycopene pathway enzymes in
Pichia pastoris
, and the manipulation of the heterobasidiomycetous yeast
Xanthophyllomyces dendrorhous
, which produces astaxanthin, a red xanthophyll with large importance in the aquaculture, pharmaceutical, and food industries. Additionally, the book includes a DNA assembler method for construction of zeaxanthin-producing strains of
Saccharomyces cerevisiae
, production of neurosporaxanthin by
Neurospora
Fusarium
, and production of torularhodin, torulene and b-carotene by
Rhodotorula
yeasts. Written in the highly successful
Methods in Molecular Biology™
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical,
provides practical experimental laboratory procedures for a wide range of carotenoids producing microorganisms.