The book has been primarily designed to help the botany students to understand the subject easily and to help them prepare for their University Examination. The present book for B.Sc. students has been written strictly according to UGC model Syllabus. The subject matter has been divided into Introduction of Plant Tissue Culture, Laboratory organization for plant tissue culture, Nutrient Media, Sterilization Methods, Cellular Totipotency, Organogenesis, Callus Culture, Plant cell suspension culture, Somatic Embryogenesis, Meristem Culture, Anther culture and Pollen Culture, Embryo culture, Protoplast culture, Somatic Hybridization and protoplast fusion, Artificial Seed Technology, Production of secondary metabolites and Application of Plant Tissue Culture .Plant tissue culture is a powerful and versatile technique that has revolutionized plant propagation, breeding, and research. This technology enables the rapid production of genetically identical plants, known as clones, with desirable traits, leading to increased efficiency in crop improvement and uniformity in commercial plant production.