The book "Problems in Mathematical Biophysics - a volume in memory of Alberto Gandolfi" aims at reviewing the current state of the art of the mathematical approach to various areas of theoretical biophysics. Leading authors in the field have been invited to contribute, having a strong appreciation of Alberto Gandolfi as a scientist and as a man and sharing his same passion for biology and medicine, as well as his style of investigation. Encompassing both theoretical and practical aspects of Mathematical Biophysics, the topics covered in this book span a spectrum of different problems, in biology, and medicine, including population dynamics, tumor growth and control, immunology, epidemiology, ecology, and others. As a result, the book offers a comprehensive and current overview of compelling subjects and challenges within the realm of mathematical biophysics. In their contributions, the authors have effectively conveyed not only their research findings but also their peculiar perspective and approach to problem-solving, dealing with oncology, epidemiology, neuro-sciences, and biochemistry. The chapters pertain to a wide array of mathematical areas such as continuous Markov chains, partial differential equations, kinetic theory, applied statistical mechanics, noise-induced transitions, and many others.