This volume is a comprehensive and up—to—date collection of strategies, reproducible methods, and prools for the in—depth analysis of Proteoglycans (PGs) and their glycan part, the GAGs. Chapters are divided into three parts detailing GAGs in biological specimens, prools for the evaluation of the in vitro and in vivo effects of PGs/GAGs, and prools for compounds related with the metabolic enzymes, epigenetic regulation, and PGs/GAGs—based inhibitors. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step—by—step, readily reproducible prools. Cutting—edge and thorough, Proteoglycans: Methods and Prools aims to provide information on the elucidated the structural and functional aspects of the complex matrix macromolecules such as the proteoglycans and glycosaminoglycans.