Excerpt from Report of the Canadian Arctic Expedition 1913-18, Vol. 4: Botany; Part A; Freshwater Algae and Freshwater Diatoms
The low temperature in these northern latitudes makes it necessary to preserve all collections in alcohol almost immediately after they have been gathered. Alcohol, as is well known, is by no means a good fixing agent for algae and, as a consequence, the algae in the collections, for the most part, were poorly fixed. The protoplasmic contents of the cells were found to be much contracted and the walls of many of the desmids collapsed. Some of the desmids had even lost their original shapes, owing to the fact that portions of their walls had become inverted. In many of the Myxophyceae the alcohol had altered the colour of the sheath and of the contents.
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