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SCREENING OF RGA MARKER(s) IN INDIAN BEAN AGAINST YMD

SCREENING OF RGA MARKER(s) IN INDIAN BEAN AGAINST YMD in Franklin, TN

Current price: $68.00
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SCREENING OF RGA MARKER(s) IN INDIAN BEAN AGAINST YMD

Barnes and Noble

SCREENING OF RGA MARKER(s) IN INDIAN BEAN AGAINST YMD in Franklin, TN

Current price: $68.00
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Size: OS

In present investigation six resistant (P-19- 169, V-19-15, V-19-35, GNIB-22, W-19-05, V-19-24) and six highly susceptible (P-19-176, W-19- 60, P-19-127, V-19-11, W-19-58, V-19-106) Indian bean genotypes were screened by nine pairs of RGA primers. Seven pairs of RGA primers were successfully amplified as a result of this in all resistant genotypes, but not in all highly susceptible genotypes. The amplicons were obtained in seven pairs of RGA primers, where five pairs of RGA primersRGA1FCG &RGA1R, VMYR1F & VMYR1R, YR4F & YR4R, RGAIB3 & RGAIB4, RGAIB5 & RGAIB6 amplified single band size of approximately 450 bp, 450 bp, 450 bp, 450 bp and 1050 bp respectively in resistant genotypes, while only two pairs of RGA primers CYR1F & CYR1R and RGAIB1 & RGAIB2 were found with prominent band of approximately 950bp and 350bp. According to the data from the current investigation, seven pairs of RGA markers have proven successful in differentiating between the resistant and highly susceptible genotypes of Indian beans. These RGA markers can be employed in investigations mapping resistance genes and validating markers with long-lasting YMV resistance.
In present investigation six resistant (P-19- 169, V-19-15, V-19-35, GNIB-22, W-19-05, V-19-24) and six highly susceptible (P-19-176, W-19- 60, P-19-127, V-19-11, W-19-58, V-19-106) Indian bean genotypes were screened by nine pairs of RGA primers. Seven pairs of RGA primers were successfully amplified as a result of this in all resistant genotypes, but not in all highly susceptible genotypes. The amplicons were obtained in seven pairs of RGA primers, where five pairs of RGA primersRGA1FCG &RGA1R, VMYR1F & VMYR1R, YR4F & YR4R, RGAIB3 & RGAIB4, RGAIB5 & RGAIB6 amplified single band size of approximately 450 bp, 450 bp, 450 bp, 450 bp and 1050 bp respectively in resistant genotypes, while only two pairs of RGA primers CYR1F & CYR1R and RGAIB1 & RGAIB2 were found with prominent band of approximately 950bp and 350bp. According to the data from the current investigation, seven pairs of RGA markers have proven successful in differentiating between the resistant and highly susceptible genotypes of Indian beans. These RGA markers can be employed in investigations mapping resistance genes and validating markers with long-lasting YMV resistance.

More About Barnes and Noble at CoolSprings Galleria

Barnes & Noble is the world’s largest retail bookseller and a leading retailer of content, digital media and educational products. Our Nook Digital business offers a lineup of NOOK® tablets and e-Readers and an expansive collection of digital reading content through the NOOK Store®. Barnes & Noble’s mission is to operate the best omni-channel specialty retail business in America, helping both our customers and booksellers reach their aspirations, while being a credit to the communities we serve.

1800 Galleria Blvd #1310, Franklin, TN 37067, United States

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