Unlike detecting constitutively expressed targets, immunohishemical detection of labile, low abundance, and short-lived signal transduction molecules can be a very difficult task. In
Signal Transduction Immunohishemistry: Methods and Prools
, IHC experts contribute detailed prools addressing the numerous challenges of signal-transduction immunohishemistry (ST-IHC). Beginning with a set of introductory chapters, the volume moves on to cover techniques used for the preservation of antigens and their unmasking, prools in digital imaging and image analysis of stained cells and tissues, high-throughput data collection and data analysis, and techniques used in neuroscience as well as cancer and stem cell research. Written in the highly successful
Methods in Molecular Biology™
series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical,
serves as an ideal guide for novices and as a bastion of inspiring ideas to be exploited by experienced researchers on the lookout for new experimental tricks and hints.