Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Methods and Prools provides a synopsis of a large array of different SILAC methods by presenting a set of prools that have been established by renowned scientists and their working groups. These include methods and prools for the labeling of various model organisms as well as advanced strategies relying on SILAC, e.g. for the analysis of protein interactions, the mapping of posttranslational modifications or the characterization of subcellular proteomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, stepby step, readily reproducible laboratory prools, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Methods and Prools will serve students and experienced scientists alike as a valuable reference of how to make use of the SILAC technology for their own research.
Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Methods and Prools provides a synopsis of a large array of different SILAC methods by presenting a set of prools that have been established by renowned scientists and their working groups. These include methods and prools for the labeling of various model organisms as well as advanced strategies relying on SILAC, e.g. for the analysis of protein interactions, the mapping of posttranslational modifications or the characterization of subcellular proteomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, stepby step, readily reproducible laboratory prools, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Methods and Prools will serve students and experienced scientists alike as a valuable reference of how to make use of the SILAC technology for their own research.
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